Environmental concerns make interest in the use of biodegradable alternatives from renewable sources. The use of cinnamon essential oil and its antimicrobial activity in the production of starch-based nanocomposite films is considered. By adding cinnamon essential oil in TiO2 films, the strain to the breaking point significantly decreased and the tensile strength increased. By simultaneously adding cinnamon essential oil and TiO2 nanoparticles, the permeability to water vapor in nanocomposite films is strongly reduced. The results of moisture absorption test confirmed the decrease of moisture absorption of nanocomposite films. In films containing 5% cinnamon essential oil, the amount of water absorption decreased by adding TiO2 nanoparticles. The synergistic effect of cyanide essential oil with Tio2 resulted in a sharp decrease in solubility in water films.Simultaneous addition of TiO2 and cinnamon has improved mechanical properties and deterioration. With increasing content of TiO2 and cinnamon, moisture absorption, solubility and permeability decreased. Also, TiO2 increased the inhibitory properties of starch films, with the increase of TiO2 concentration decreasing the moisture absorption, WVP and solubility and increasing the angle of contact. The research findings indicated that the functional properties of starch films can be improved by adding the combined CEO and TiO2.