Pesticide residues and their derivatives in agricultural products are a serious threat to consumers' health. The aim of this study was to evaluate the amount of pesticide residues in Berberis vulgaris fruits by extraction and extraction methods in Iran. In general, barberry fruit samples from Berberis vulgaris gardens from 6 different regions were used in which 3 diazinon, fuzalone and chloropyraysus pesticides were used. In this study, two extraction and extraction methods were used. The conventional method of hydroalcoholic extraction and quaternary extraction method (QuEChERS) is a rapid, easy, efficient, reliable, and multi-residue extraction method) followed by GC-MS gas chromatography (GC-MS) spectrometry. Residues from pesticides were not detectable in any of the samples prepared by the first method, while the samples obtained from the second method indicated that 11% of the samples were contaminated with diazinon, 5% with chlorpyrifos and 5.5% Fuzalone. The results indicate the remaining amount of these pesticides in berberis vulgaris fruit in Iran, which requires regular monitoring of pesticides using updated extraction methods.